Gene cloning of anthocyanin synthase in Fagopyrum dibotrys and correlation between its expression level and anthocyanin content
10.7501/j.issn.0253-2670.2014.07.017
- Author:
Xing-Xing BU
1
Author Information
1. College of Life Sciences, Sichuan Agricultural University
- Publication Type:Journal Article
- Keywords:
Anthocyanin;
Anthocyanin synthase;
CDNA sequence;
Fagopyrum dibotrys (D. Don) Hara;
Gene cloning
- From:
Chinese Traditional and Herbal Drugs
2014;45(7):985-989
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To obtain the full-length cDNA sequence of anthocyanins synthase (ANS) gene from Fagopyrum dibotrys (FdANS), and to analyze the expression of FdANS in each tissue and the total anthocyanin content during florescence of F. dibotrys. Methods: The cDNA sequence of ANS gene was cloned by homology cloning from F. dibotrys. The expression of ANS was analyzed by semi-quantitative RT-PCR. The content of total anthocyanin was measured by spectrophotometry. Results: The open reading frame (ORF) of FdANS was 1077 bp and encoded a protein with 358 amino acids named FdANS. Bioinforamtion analysis suggested that the amino acid sequence of FdANS had the higher homology with those in other plants. Both the gene expression FdANS in different tissues during florescence of F. dibotrys and the total anthocyanin content were in the order of flowers > leaves > stems> roots. Conclusion: The cDNA sequence of FdANS is firstly obtained from F. dibotrys and its coding protein has the typical characteristic of ANS homologous protein. The gene expression of FdANS shows the correlation to the total anthocyanins content in the flowers, leaves, stems, and roots of F. dibotrys.
