Simultaneous determination of 11 components in Zhizi Jinhua Pill by UPLC
10.7501/j.issn.0253-2670.2014.07.011
- Author:
Xiao-Hu CHEN
1
Author Information
1. Chongqing Institute for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
Baicalin;
Berberine;
Chlorogenic acid;
Emodin;
Geniposide;
UPLC;
Zhizi Jinhua Pill
- From:
Chinese Traditional and Herbal Drugs
2014;45(7):955-959
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a UPLC method for the simultaneous determination of 11 components, such as chlorogenic acid, geniposide, baicalin, berberine, wogonoside, baicalein, aloe-emodin, wogonin, emodin, chrysophanol, and physcion in Zhizi Jinhua Pill (ZJP). Methods: The chromatographic separation was achieved on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile (A)-0.1% phosphate acid solution (B) as mobile phase at the flow rate of 0.4 mL/min for gradient elution; The column temperature was 35°C. The determination wavelengths were 324 nm for chlorogenic acid, 238 nm for geniposide, 345 nm for berberine, 276 nm for baicalin, wogonoside, baicalein, and wogonin, and 254 nm for aloe-emodin, emodin, chrysophanol, and physcion. Results: The 11 compounds were well separated. The RSD values of precision and reproducibility were all less than 2%. The stability was good in 24 h. The linear relationship between the concentration and peak areas of the 11 compounds was all linear (r ≥ 0.9995). The average recoveries were between 98.68%-100.47% and the RSD values were all less than 1.5%. Conclusion: The method is simple, reliable, and accurate, and could be used for the quality control of ZJP.
