Analysis on chemical constitutes in Psoraleae Fructus by combination of HPLC/TOF-MS and HPLC/IT-MSn
10.7501/j.issn.0253-2670.2014.07.006
- Author:
Juan-Min LUO
1
Author Information
1. Technology Center of China Tobacco Jiangxi Industrial Co., Ltd.
- Publication Type:Journal Article
- Keywords:
HPLC;
Isopsoralen;
Multi-component analysis;
Psoraleae Fructus;
Psoralen;
TOF-MS
- From:
Chinese Traditional and Herbal Drugs
2014;45(7):924-928
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establsh a combinative HPLC/TOF-MS and HPLC/IT-MSn method for quantitative analysis on chemical constituents in Psoraleae Fructus. Methods: Separation was performed on a Phenomenex Luna C18 (5 μm) reverse-phase column. The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute. The flow rate was 1 mL/min. A combination of time-of-flight mass spectrometer (TOF-MS) and ion trap tandem mass spectrometer (IT-MSn) was applied for qualitative analysis under positive and negative ion mode. Results: Under optimized LC/MS condition, the molecular formulae of 13 components were screened by TOF-MS and all of them were then identified by IT-MSn. Conclusion: The established combinative HPLC/MS method could thus be considered as an effective and credible pattern for the analysis on the multi-components of Chinese materia medica and it also could be used for as the analysis on other complex systems.
