Mechanism of Chinese herbal ingredients in Xinhuang Tablet on attenuating gastrointestinal toxicity induced by indomethacin
10.7501/j.issn.0253-2670.2014.08.014
- Author:
Jing LIU
1
Author Information
1. Tianjin Institute of Pharmaceutical Research
- Publication Type:Journal Article
- Keywords:
Attanuation;
Gastric ulcer;
Gene chip;
Indomethacin;
Xinhuang Tablet
- From:
Chinese Traditional and Herbal Drugs
2014;45(8):1115-1120
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the mechanism of Chinese herbal ingredients in Xinhuang Tablet on attenuating gastrointestinal toxicity induced by indomethacin. Methods: Xinhuang Tablet and a considerable dose of indomethacin were ig given to rats once a daily, lasted for 3 d, and then the levels of gastric acid, the activity of gastric protease, and the indexes of the serum gastrin, COX-1, COX-2, etc were determined. Xinhuang Tablet and a considerable dose of indomethacin were ig given to rats once daily, lasted for 2 weeks, and then the liver tissue gene expression microarray analysis was applicated to research the effects of Xinhuang Tablet and indomethacin on the gastrointestinal toxicity associated proteins. The mechanism of Chinese herbal ingredients in Xinhuang Tablet on attenuating the gastrointestinal toxicity induced by indomethacin was explored on the gene level. Results: Indomethacin could promote gastrin secretion, thus increasing gastric acid secretion and the vitality of pepsin. Xinhuang Tablet could significantly inhibit gastrin secretion, the activity of pepsin, and gastric acid secretion. Indomethacin could significantly inhibit the vitality of COX-1, while Xinhuang Tablet could increase the COX-1 vitality and further protect the gastric mucosa. Indomethacin could significantly up-regulate the gene expression of perforin, while down-regulate the gene expression of Mmp2 as well as the related protein genes of immune system and coagulation system. Compared with indomethacin, Xinhuang Tablet could significantly lower the expression of perforin gene, and raise the expression of Mmp2, Cldn1, the immune-related protein genes, coagulation factors, and fibrinogen. Conclusion: These results indicated that the attenuating mechanism of Chinese herbal ingredients in Xinhuang Tablet on the gastrointestinal toxicity of indomethacin might be ralated to the inhibition of gastrin secretion, improved vitality of inhibited COX-1 by indomethacin, down-regulate perforin, up-regulate Cldn1, immune-related protein genes, coagulation factors, fibrinogen, increase the membrane stability, and promote wound healing as well.
