Comparison on extracting methods of total alkaloids in Crinum asiaticum seeds
10.7501/j.issn.0253-2670.2014.08.010
- Author:
Miao YU
1
Author Information
1. Center of Life Science and Environmental Science, Harbin University of Commerce
- Publication Type:Journal Article
- Keywords:
Crinum asiaticum L.;
Heating reflux extraction;
Response surface methodology;
Total alkaloids;
Ultrasonic extraction
- From:
Chinese Traditional and Herbal Drugs
2014;45(8):1089-1095
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the extracting methods and technology for total alkaloids from the seeds of Crinum asiaticum. Methods: Ultrasonic extraction and reflux extraction were used to extract the total alkaloids from the seeds of C. asiaticum. Results: The two results showed that the extracting rate of alkaloids by ultrasonic extraction was 0.4357% under ultrasonic temperature of 58°C, ultrasonic time of 47 min, ultrasonic power of 600 W, percentage of ethanol volume of 95%, solid-liquid ratio of 1:6, and extraction for three times. Additionally, the extracting yield of alkaloids by reflux extraction was 0.4494% under extraction temperature of 84°C, extraction time of 2.5 h, solid-liquid ratio of 1:9, percentage of ethanol volume of 95%, and extraction for three times. Conclusion: Considering the extracting rate of alkaloids, operating methods, and the difficulty of removing impurity, the heating reflux extraction is an ideal method for total alkaloids extracting from the seeds of Crinum asiaticum.
