Representation and in vitro transdermal characteristics of glycyrrhizic acid nanoparticles prepared by antisolvent recrystallization method
10.7501/j.issn.0253-2670.2014.08.006
- Author:
Wen-Jia XU
1
Author Information
1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University
- Publication Type:Journal Article
- Keywords:
Antisolvent recrystallization;
Cutaneous penetration;
Glycyrrhizic acid;
Nanoparticles;
Release rate
- From:
Chinese Traditional and Herbal Drugs
2014;45(8):1068-1071
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare glycyrrhizic acid nanoparticles (GAN) using ethanol as solvent and ethyl acetate as the antisolvent, and to investigate the osmosis of GAN as carrier of dermal administration. Methods: Using scanning electron microscope (SEM), laser particle size analyzer, Fourier transform-infrared spectroscopy (FT-IR), and release rate analysis, the untreated glycyrrhizic acid (GA) and GA powder were characterized; In vitro cutaneous permeation experiments were carried out on modified Franz diffusion cells, using excised mouse skin. The concentration of diammonium glycyrrhizinate in the receptor compartments and skin were determined by HPLC. Results: The GAN was spherical, the average particle size was 220 nm, and the dissolution rate of nanosized glycyrrhizinate was improved obviously. The transdermal rate of nanosized glycyrrhizinate was better than the original glycyrrhizin, and the 12 h unit accumulation transmissibility of GAN and GA was 78.51 and 9.792 μg/cm2, respectively. Conclusion: After preparing, the particle size becomes smaller, the chemical structure does not change significantly, and both of dissolution rate and in vitro transdermal performance have been improved. The GAN has the potential application values in the development of pharmaceutical industry.
