Optimization of preparation technology of chrysophanol-glucan complex based on central composite design and response surface methodology
10.7501/j.issn.0253-2670.2014.11.008
- Author:
Zhen WU
1
Author Information
1. Chongqing Academy of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
Central composite design;
Chrysophanol;
Glucan complex;
Response surface methodology;
Solubilization
- From:
Chinese Traditional and Herbal Drugs
2014;45(11):1540-1544
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To improve the solubility and bioavailability of chrysophanol (Chr), the preparation of inclusion complex of Chr and β-glucan (Chr-β-glu) was optimized by central composite design and response surface methodology. Methods: The inclusion complex of Chr-β-glu, prepared by electromagnetic stirring and spray drying methods (ES), was optimized by central composite design. Using the mass ratio of β-glu and Chr, temperature, and stirring rate as independent variables and using the inclusion rate as dependent variable, the multiple linear regression and binomial equations were fitted to the data of overall variables, and the resulting equation was used to produce 3-D response surface graphs. The optimal formulation was predicted and the best prescription process was chosen to verification test. Results: The correlation coefficient of second-order quadratic model was high and the prediction was good (R2=0.967 2). The optimal conditions for the preparation of Chr-β-glu were as follows: material proportion was 1:2.8, temperature was 52.0°C, and stirring rate was 570.9 r/min. The maximum inclusion rate predicted by the model was 40.6% and the actual inclusion rate was (41.4 ± 0.81)%. Conclusion: Central composite design and response surface methodology are successfully used to optimize the preparation of Chr-β-glu. The optimized process is reliable, stable, and available for the industrial production.
