Effect of active fraction from Huanglian Jiedu Decoction on activation of astrocytes and expression of Cx43 in cerebral ischemic penumbra
10.7501/j.issn.0253-2670.2014.13.013
- Author:
Jian ZHANG
1
Author Information
1. College of Traditional Chinese Medicine, Capital Medical University
- Publication Type:Journal Article
- Keywords:
Active fraction;
Astrocytes;
Cerebral ischemia;
Connexin 43;
GFAP;
Huanglian Jiedu Decoction;
Ischemic penumbra
- From:
Chinese Traditional and Herbal Drugs
2014;45(13):1876-1882
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of water extract and active fractions from Huanglian Jiedu Decoction (HJD) (total alkaloids, total favonoids, and total iridoids) on the activation of astrocytes and expression of connexin 43 (Cx43) in ischemic penumbra of rats after focal cerebral ischemia. Methods: The rat model of middle cerebral artery ischemia was established using suture-occluded method. The male SD rats were randomly divided into Sham-operation, model, water extract of HJD (800 mg/kg), total alkaloids (44 mg/kg), total flavonoids (50 mg/kg), and total iridoids (80 mg/kg) groups. They were ig administered for continuous 7 d, once daily. The histopathologic changes of brain in rats were observed by HE staining. The expression of GFAP and Cx43 was detected by immunofluorescence assay and RT-PCR was used to detect the gene expression of Cx43. Results: In the model group, the number of neurons in ischemic penumbra of rats was significantly decreased and the expression of GFAP, Cx43, and Cx43 protein was obviously increased compared with Sham-operation group (P<0.01). The three active fractions from HJD (total alkaloids, total favonoids, and total iridoids) could increase the number of neurons in the ischemic penumbra of rats and decrease the expression of GFAP, Cx43, and Cx43 protein obviously compared with the model group (P<0.05, 0.01). Conclusion: The active fractions (total alkaloids, total favonoids, and total iridoids) could inhibit the over activation of astrocytes and the expression of Cx43, so as to protect the neurons in ischemic penumbra.
