Optimization of preparation of volatile oil from Houttuynie Herba-β-cyclodextrin inclusion complex by central composite design-response surface method
10.7501/j.issn.0253-2670.2014.13.009
- Author:
Ying LI
1
Author Information
1. The Second People's Hospital of Fujian University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Central composite design;
Inclusion process;
Response surface method;
Volatile oil prepared from Houttuyniae Herba;
β-cyclodextrin
- From:
Chinese Traditional and Herbal Drugs
2014;45(13):1855-1862
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the preparation process of volatile oil prepared from the aerial parts of Houttuynia cordata (Houttuynie Herba, HH)-β-cyclodextrin (β-CD) inclusion complex by central composite design-response surface method. Methods: To prepare the inclusion complex by stiring, with the overall desirability (OD) values of inclusion ratio, yield of the inclusion complex, and methyl-n-nonyl-ketone content as indexes, the influence of inclusion temperature, inclusion time, and ratio of β-CD-HH volatile-oil to inclusion process was investigated by response surface method, the results were fitted by multi-linear equation and second-order polynomial equation, and the optimal inclusion process was predicted according to formulation. The quality of inclusion complex was evaluated by IR and HS-SPME-GC-MS. Results: The optimal inclusion technologies of the HH volatile oil-β-CD inclusion complex were as follows: inclusion time 4.8 h, inclusion temperature 62°C, and ratio of β-CD-HH volatilel oil 7.79:1. Bias of inclusion ratio, yield of the inclusion complex, and methyl-n-nonyl-ketone content between observed and predicted values were 1.70%, 1.49%, and 2.22% respectively. Conclusion: Using the central composite design-response surface method to optimize the preparation of HH volatile oil-β-CD inclusion complex has a good prediction.
