Metabolic distribution and stability of cucurbitacin C in Cucumber sativus
10.7501/j.issn.0253-2670.2014.14.023
- Author:
Zhi-Xing QING
1
Author Information
1. National Herbal Medicine Production Technology Center, Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cucumber sativus L.;
Cucurbitacin C;
HPLC-Q-TOF-MS;
Metabolic distribution;
Stability
- From:
Chinese Traditional and Herbal Drugs
2014;45(14):2080-2083
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the metabolic distribution of cucurbitacin C in different parts of cucumber plant and investigate the change of cucurbitacin C in the leaves of Cucumber sativus. with different treatments. Methods: The content of cucurbitacin C in each part of the whole plant, as well as in the fresh leaves treated by drying at 100°C and room temperature, was determined by an HPLC-DAD method established for the quantification of cucurbitacin C. And the transformation of cucurbitacin C was investigated by an HPLC-Q-TOF-MS method Results: The content of cucurbitacin C in the fresh leaves was found at the highest level, but decreased along with the leaves turned old. It also reduced gradually from the top to the base of the stems. Cucurbitacin C was not detected in the roots, leafstalks, female flowers, and fruits. The content level decreased significantly with drying at either 100°C or room temperature. The possible reason was that cucurbitacin C transformed to cucurbitacin glycoside, tentatively identified as 3-glu-cucurbitacin C, when the fresh leaves drying in room temperature. Conclusion: Cucurbitacin C is an unsteadiness substance in C. sativus. Fresh leaves are an ideal resource of obtaining this compound.
