Effect of monocrotaline on induction of pancreatic cancer cell BxPC-3 into polyploid giant cells
10.7501/j.issn.0253-2670.2014.14.016
- Author:
Jun-Ying ZENG
1
Author Information
1. Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cyclin B1;
Monocrotaline;
Pancreatic cancer cells;
Polyploid giant cells
- From:
Chinese Traditional and Herbal Drugs
2014;45(14):2042-2046
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of monocrotaline (MCT) extract from Crotalaria sessiliflora on inducing human pancreatic cancer cell BxPC-3 into polyploid giant cells in vitro. Methods: BxPC-3 cells (1 × 104/mL) were inoculated with MCT (0, 5, 10, and 20 μg/mL) in RPMI-1640 for 72 h, respectively, then the cell morphology was observed by Giemsa staining, and the DNA content was measured by flow cytometry; BxPC-3 cells-induced apoptosis was checked by AnnexinV-FITC/PI double staining using flowcytometry, BxPC-3 polyploid giant cell genome was checked by chromosome spreading assay, and Cyclin B1 expression also was analysed by Western blotting. Results: Giemsa staining and DNA content by flow cytometry showed that MCT induced BxPC-3 cell into polyploid giant cells in vitro. MCT treatment for 72 h appeared 4N, and 8N polyploid giant of BxPC-3 cells, induction of apoptosis, decreased the expression of cyclin B1 in a dose dependent manner. Chromosome analysis demonstrated once again that polyploid giant cell was mainly in 8N. Conclusion: Monocrotaline might exert its antitumor effect by blocking the cell cycle, inducing apoptosis, and down-regulating Cyclin B1 protein.
