Chemical constituents from stems of Syzygium jambos var. jambos and their in vitro cytotoxicity
10.7501/j.issn.0253-2670.2014.14.006
- Author:
Da-Du LIN
1
Author Information
1. Key Laboratory of Chinese Medicinal Resource from Lingnan (Ministry of Education), Research Center of Medicinal Plant Resource Science and Engineering, Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Alphitolic acid;
Asiatic acid;
Cytotoxicity;
Syzygium jambos var. jambos (L.) Alston;
Ursolic acid;
β-sitosterol
- From:
Chinese Traditional and Herbal Drugs
2014;45(14):1993-1997
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents from the stems of Syzygium jambos var. jambos. Methods: The phyotchemicals were isolated by using various chromatographic techniques such as silica gel, Sephadex LH-20, alumina column chromatography, and preparative HPLC process. The structures were elucidated on the basis of spectral data analysis. Their cytotoxicity against human liver cancer cells Huh-7 was conducted using MTT assay. Results: Twelve compounds were isolated from the EtOAc and MeOH extracts in the stems of S. jambos and identified as alphitolic acid (1), urolithin A (2), dibutyl phthalate (3), betulinic acid (4), ursolic acid (5), 1-(4-methoxyphenyl)-1, 2-propanediol (6), 2, 6-dimethoxy-1, 4-benzoquinone (7), diisobutyl phthalate (8), β-sitosterol (9), 3-acetyl-ursolic acid (10), asiatic acid (11), and arjunolic acid (12), respectively. All tested compounds 1, 7, 10, and 11 + 12 mixture did not show the cytotoxic activities towards human tumor cells Huh-7 in the test range up to 50 μmol/L. Conclusion: Except compound 4, the other 11 compounds are obtained from this plant for the first time. All tested compounds exhibit no significant cytotoxic activity against Huh-7 cells.
