Effect of Gridi-13 on expression of NSE, TNF-α, and IL-6 after focal cerebral ischemia in rats
10.7501/j.issn.0253-2670.2015.05.017
- Author:
Cai-Yun TIAN
1
Author Information
1. Institute of Cardiovascular, Baotou Medical Collage
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Gridi-13;
Neuron-specific enolase;
Tumor necrosis factor-αInterleukins-6
- From:
Chinese Traditional and Herbal Drugs
2015;46(5):716-720
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the roles of neuron-specific enolase (NSE), tumor necrosis factor-α (TNF-α), and interleukins-6 (IL-6) in serum on focal cerebral ischemic injury of rats as well as the effect of Gridi-13 on them. A total of 360 male SD rats were selected. They were randomly divided into model group, Sham-operated group, Gridi-13 large dose group, Gridi-13 middle dose group, and Gridi-13 small dose group. Each group was randomly divided into 1, 6, 12, 24, 72, and 120 h time phase groups. The middle cerebral artery occlusion (MCAO) model was established by modified Zea Longa thread-occlusion method and the contents of NSE, TNF-α, and IL-6 in serum were determined by ELISA method. The expression of NSE, TNF-α, and IL-6 was very weak in Sham-operated group while rising after the cerebral ischemic injury for 1 h compared with the model group. The peak values of NSE, TNF-α, and IL-6 appeared at 12, 24, and 24 h, respectively and then going down gradually (P < 0.01). Gridi-13 in each dose group could predominantly reduce the nervous function score and the expression levels of NSE, TNF-α, and IL-6 were remarkably inhibited (P < 0.01 and P < 0.05). Garidi-13 has the significant protection on the cerebral ischemia injury in rats, which may be related with alleviating neuronal damage, regulating inflammatory factors, and relieving the injury of brain tissue, so as to play a role of therapeutic action in cerebral ischemic injury of rats.
