Establishment of skin and blood microdialysis sampling method of sinomenine and triptolide in vivo
10.7501/j.issn.0253-2670.2015.14.010
- Author:
Zhou LI
1
Author Information
1. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Blood microdialysis;
Recovery in vitro;
Sinomenine;
Skin microdialysis;
Triptolide
- From:
Chinese Traditional and Herbal Drugs
2015;46(14):2076-2081
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish skin and blood microdialysis (MD) method of sinomenine and triptolide. Methods: Incremental method was applied and the recovery rate of sinomenine and triptolide was used as an index to investigate the impact of perfusion fluid flow rate to the recovery (R). The flow rate and sampling interval were then determined according to the R and actual operation; The reduction method and incremental method were applied in the in vitro study to investigate impact of perfusate concentration to the R and a transfer rate (D) and the proportional relationship between them; Reduction method was applied in the in vivo study to investigate and determine the stability of the probe recoveries. Results: The recovery of skin and blood probes in vitro of sinomenine and triptolide increased with the flow rate in the range of 0.5-2.5 μL/min. The flow rate was determined to be 1 μL/min and the sampling interval was determined to be 0.5 h; The R of sinomenine and triptolide in skin and blood probes in vitro and D was stable and equal despite of change of sinomenine concentration in 10-40 μg/mL and triptolide concentration in 3-12 μg/mL, indicating that the effect of drug concentration on the MD probe R was small and that R could be replaced by the D; The D in vivo using MD method was measured at 10 h. The D values of sinomenine skin and blood probe were (41.27 ± 0.87)% and (37.8 ± 0.99)%, respectively and those of triptolide were (44.68 ± 1.28)% and (51.35 ± 1.15)%, respectively, indicating that the D of sinomenine and triptolide was stable within 10 h. Conclusion: The established method of sinomenine and triptolide in MD can be applied to investigate the kinetics in skin and blood after sc administration of formulation.
