UFLC fingerprint of Huoxiang Zhengqi Dripping Pill
10.7501/j.issn.0253-2670.2015.15.012
- Author:
Yong-Zhi ZHANG
1
Author Information
1. School of Pharmacy, Shenyang Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Angelicae Dahuricae Radix;
Atractlodis Rhizoma;
Citri Reticulatae Pericarpium;
Fingerprint;
Huoxiang Zhengqi Dripping Pill;
Magnoliae Officmalis Cortex;
Quality control;
Similarity;
UFLC-IT-TOF/MS
- From:
Chinese Traditional and Herbal Drugs
2015;46(15):2246-2250
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the ultra-fast liquid chromatographic (UFLC) fingerprint of Huoxiang Zhengqi Dripping Pill (HZDP) for evaluating the quality of the preparation. Methods: The UFLC separation was achieved on an Waters Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile (A)-0.2% formic acid solution (B) as mobile phase at the flow rate of 0.4 mL/min by gradient elution of 0-6 min, 10% →15% A, 6-9 min, 15% →20% A, 9-14 min, 20% →30% A, 14-19 min, 30% → 50% A, 19-21 min, 50% →65% A, 21-26 min, 65% →75% A, 26-28 min, 75% A, 28-30 min, 75% →10% A, 30-32 min, 10% A. The column temperature was 30 ℃. The UV detection wavelength was 300 nm. The similarity of 11 batches of HZDP was analyzed with similarity evaluation system for chromatographic fingerprint of tradition Chinese medicine (Version 2004 A). Results: Fingerprints of 11 batches of HZDP were established and 18 common peaks were identified, 13 of which belonged to herbs and were identified by the reference substance and UFLC-IT-TOF/MS. Among them, five common peaks (peaks 6, 7, 9, 10, and 12) came from Angelicae Dahuricae Radix, three common peaks (peaks 4, 5, and 8) came from Citri Reticulatae Pericarpium, four common peaks (peak 1, 2, 11, and13) came from Magnoliae Officmalis Cortex, and one common peak (peak 17) came from Atractlodis Rhizoma. Conclusion: The method is rapid, accurate, reliable, and reproducible. The established fingerprint could provide the references for the study of substance basis and quality control of HZDP.
