Determinatoin of macromolecule protein in Chinese materia medica injection
10.7501/j.issn.0253-2670.2015.15.009
- Author:
Xue WANG
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Chinese materia medica injection;
Coomassie brilliant blue method;
Lowry method;
Protein;
Size exclusion chromatograph method;
Sulfosalicylic acid method
- From:
Chinese Traditional and Herbal Drugs
2015;46(15):2228-2231
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the feasibility of four methods for macromolecule protein determination in Chinese materia medica (CMM) injection. Methods: The sensitivity, specificity, and protein test results of sulfosalicylic acid, Coomassie brilliant blue, Lowry, and size exclusion chromatograph (SEC) methods on five kinds of CMM injections were compared. Results: Sulfosalicylic acid method had low sensitivity, Coomassie brilliant blue and Lowry methods had poor specificities, and SEC method had high sensitivity and good specificity. No protein was detected in five kinds of CMM injection by SEC. Conclusion: SEC is suitable for the determinatoin of macromolecule protein in CMM injection.
