Determination of notoginsenoside R1, ginsenoside Rg1, Rb1, and scutellarin in Baogan Pill by RP-HPLC
10.7501/j.issn.0253-2670.2015.16.013
- Author:
Hong-Sheng LIU
1
Author Information
1. Key Laboratory of Emergency Medicine of Critical Disease of Healthful Ministry in First Central Hospital
- Publication Type:Journal Article
- Keywords:
Baogan Pill;
Ginsenoside Rb1;
Ginsenoside Rg1;
Notoginsenoside R1;
RP-HPLC;
Scutellarin
- From:
Chinese Traditional and Herbal Drugs
2015;46(16):2417-2420
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an RP-HPLC method for determining the mass concentration of notoginsenoside R1, ginsenoside Rg1, Rb1, and scutellarin in Baogan Pill, and to provide the quality guarantee for Baogan Pill. Methods: Agilent Zorbax SB-C18 (150 mm×46 mm, 5 μm) column were used. Acetonitrile and 0.1% phosphoric acid water-solution were used as mobile phase of gradient elution, the volume flow was at 1.0 mL/min; Ultraviolent determination wavelength was 203 nm; Column temperature was at 30℃, and injection volume was 5 μL. Results: The lowest detection limit in notoginsenoside R1 was 0.287 mg/L, the linear range was 4.427-141.668 mg/L; The lowest detection limit in ginsenoside Rg1 was 0.182 mg/L, the linear range was 6.055-193.75 mg/L; The lowest detection limit in ginsenoside Rb1 was 0.182 mg/L, the linear range was 3.255-104.167 mg/L; The lowest detection limit in scutellarin was 0.305 mg/L, the linear range was 5.729-183.333 mg/L. The average recovery of notoginsenoside R1, ginsenoside Rg1, Rb1 and scutellarin in Baogan Pill respectively were 101.76%, 99.66%, 99.43%, and 101.26%; The precision RSD values respectively were 1.81%, 1.79%, 1.39%, and 1.51%; The repeatability RSD values respectively were 1.71%, 1.86%, 0.97%, and 1.63%; The stability RSD values respectively were 1.62%, 1.25%, 1.10%, and 1.41%. The average amounts of notoginsenoside R1, ginsenoside Rg1, Rb1, and scutellarin in the selected samples per pill respectively were 4.303, 31.729, 20.776, and 1.071 μg. Conclusion: The method is believable for determining the mass concentration of R1, Rg1, Rb1, and scutellarin with its simplicity, sensibility, repeatability, and better recovery rate.
