Isolation and quantitative determination of polyacetylenes in Bupleuri Radix
10.7501/j.issn.0253-2670.2015.16.004
- Author:
Yuan FANG
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University
- Publication Type:Journal Article
- Keywords:
Bupleuri Radix;
Bupleurynol A;
Bupleurynol C;
Polyacetylenes;
Quantitative determination;
UPLC
- From:
Chinese Traditional and Herbal Drugs
2015;46(16):2365-2370
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the separation of polyacetylenes and content of them in Bupleuri Radix from different bases and different habitats. Methods: Silica gel, Sephadex LH-20 column chromatography, and preparative thin layer chromatography were used for the isolation and purification, the chemical structures were identified by NMR spectroscopic analyses, and the content of the polyacetylenes was determined by UPLC-PDA. Results: Four polyacetylenes were isolated from the petroleum ether fraction of Bupleuri Radix and identified as (2Z, 8Z, 10E)-pentadecatriene-4, 6-diyn-1-ol (1), (2Z, 8E, 10E)-pentadecatriene-4, 6-diyn-1-ol (2), (2Z, 8Z, 10E)-heptadecatriene-4, 6-diyn-1-ol (3), and bupleurynol (4). The contents of the polyacetylenes in Bupleuri Radix from various cultivation locations and origins were different, the locations may have greater impacts on the content than the species. Conclusion: Compounds 1 and 3 are two new natural products named bupleurynol A and bupleurynol C, and the two compounds are more stable in the presence of Cis-trans isomers. The method is easy, accurate, reliable, and has the good reproducibility and high sensitivity, and can be used as a detection method for the polyacetylenes in Bupleuri Radix.
