Pharmacokinetic study on puerarin and ferulic acid alone or their combination used in rats with focal cerebral ischemia
10.7501/j.issn.0253-2670.2015.18.016
- Author:
Rong-Bing YANG
1
Author Information
1. Cerebrovascular Disease Research Institute, Zhejiang Chinese Medicine University
- Publication Type:Journal Article
- Keywords:
Combination administration;
Ferulic acid;
Model of focal cerebral ischemia;
Pharmacokinetics;
Puerarin
- From:
Chinese Traditional and Herbal Drugs
2015;46(18):2759-2763
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the pharmacokinetic changes of puerarin and ferulic acid alone or their combination used in rats with focal cerebral ischemia and to analyze their mutual influence. Methods: Twenty-four SD rats were randomly divided into three groups such as puerarin (50 mg/kg) group, ferulic acid (50 mg/kg) group, and combination (50 mg/kg + 50 mg/kg) group. The middle cerebral artery occlusion (MCAO) model was established. The model rats were iv injected with puerarin and ferulic alone, and then the blood was collected at different time points. The HPLC-DAD method was applied to determining the plasma concentration of puerarin and ferulic acid at different time points and the drug-time curve was drawn. The DAS 3.2.6 and SPSS 19.0 softwares were used to analyze the data. Results: The pharmacokinetic parameters: AUC, t1/2, Cmax, and AUMC had no significant difference (P > 0.05), while the clearance of puerarin group had the significant difference with that of the combination group and mean residence time (MRT) of combination group was more extended than that of ferulic acid group. Conclusion: In rats with focal cerebral ischemia, the combination of puerarin and ferulic acid could significantly extend the MRT in rats, which reflects the slow-release effect between drug-drug interaction.
