Effect of Polygonatum sibiricum polysaccharides on inflammatory raction mediated by NF-κB and myocardium tissue morphology in acute myocardial infarction rats
10.7501/j.issn.0253-2670.2015.18.014
- Author:
Li LI
1
Author Information
1. College of Pharmacy, Anhui University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Acute myocardial infarction;
IL-6;
Inflammatory reaction;
NF-κB;
Polygonatum sibiricum polysaccharides;
TNF-α
- From:
Chinese Traditional and Herbal Drugs
2015;46(18):2750-2754
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of Polygonatum sibiricum polysaccharides (PSP) on the inflammatory raction mediated by NF-κB and myocardium tissue morphology in acute myocardial infarction (AMI) rats, and to explore the myocardial protective effect and its mechanisms. Methods: The AMI model was established by closing the left anterior descending coronary artery of SD rats, Sham group threading without ligation, then the model rats were randomly divided into model group, high-, mid-, low-dose PSP (900, 450, and 225 mg/kg) groups, and positive group (Shexiang protect myocardial pills, 12.2 mg/kg), with 10 rats in each group, ig administered once daily for 28 d. ECG S-T changes were measured before surgery, immediately after surgery, and 1, 3, 7, 10, 14, 21, 28 d after surgery in each group; The levels of inflammatory cytokines IL-6, TNF-α, NF-κB, CKMB, and LDH in serum were measured by ELISA; The myocardial tissue morphology was measured by HE staining. Results: Compared with the model group, PSP inhibited S-T segment elevation of ECG in AMI rats; The serum levels of TNF-α, IL-6, NF-κB, CKMB, and LDH in high- and mid-PSP groups decreased significantly (P < 0.05, 0.01), and the degree of myocardial injury was significantly improved. Conclusion: PSP could improve the myocardial injury, reduce the inflammation, and repair the ischemic myocardium in AMI rats. The mechanisms may be related to regulating the inflammatory raction mediated by NF-κB.
