Inhibition of epipinoresinol on macrophage foam and potential mechanisms
10.7501/j.issn.0253-2670.2015.18.012
- Author:
Shuai WANG
1
Author Information
1. Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Cholesterol efflux;
Cholesterol influx;
Epipinoresinol;
Foam cell;
Macrophage
- From:
Chinese Traditional and Herbal Drugs
2015;46(18):2737-2742
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clarify the potential inhibitive effect of epipinoresinol on macrophage foam and potential mechanisms. Methods: The inhibition of epipinoresinol on foam cell formation after stained with oil red O was assessed by Image-Pro Plus and Microplate Reader, and the effect on cholesterol efflux and influx was tested by fluorescence detection to obtain cholesterol inflows-time curve and outflow rate. Additionally the cholesterol flow-associated genes expression was checked by real-time PCR (RT-PCR). Results: Epipinoresinol dose-dependently inhibited the enhanced cholesterol accumulation elicited by oxidized low-density lipoprotein cholesterol (ox-LDL) in RAW264.7 cells. Treatment with epipinoresinol significantly enhanced the cholesterol efflux mediated by high-density lipoprotein (HDL) and substantially inhibited the cholesterol influx. RT-PCR showed that epipinoresinol significantly increased the mRNA levels of PPARγ, LXRα, ABCA1, and ABCG1, decreased those of SR-A1 and SR-A2. Conclusion: Epipinoresinol is a new inhibitor on foam cell formation that may stimulate the cholesterol efflux through up-regulating the PPARγ-LXRα-ABCA1/ABCG1 pathway and prevent cholesterol influx through down-regulating SR-A1 and SR-A2, which may be useful on atherosclerosis treatment.
