Preparation and in vitro release of lyophilized powder of baicalein solid lipid nanoparticles
10.7501/j.issn.0253-2670.2015.18.009
- Author:
Tong YU
1
Author Information
1. College of Pharmacy, Liaoning Medical University
- Publication Type:Journal Article
- Keywords:
Baicalein;
In vitro release;
Lyophilized powder;
Solid lipid nanoparticles;
Solvent emulsification evaporation method
- From:
Chinese Traditional and Herbal Drugs
2015;46(18):2720-2726
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare lyophilized powder of baicalein solid lipid nanoparticles (BA-SLNs) and investigate its physicochemical properties and release characteristics. Methods: Ba-loaded SLN was prepared by solvent emulsification-evaporation method, the formulation was optimized by orthogonal design, with encapsulation efficiency (EE) as reference, the measurement of particle size, morphology, Zeta potential, the polydispersity index (PDI), and in vitro drug release behavior. The lyophilized powder of appearance, color, redispersibility as indexes, the differential scanning calorimetry (DSC), X-Ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) were used to analyze its material phase of the drug in nanoparticles. Results: The Ba-SLNs assumed a spherical shape with an even distribution of diameter and particle size was (82.64 ± 6.78) nm, the PDI was 0.242 ± 0.013, Zeta potential was (-25.7 ± 0.5) mV, EE was (81.3 ± 1.2)%, and drug loading was (7.16 ± 0.14)% (n = 3). The 5% mannitol was the best protective agent for lyophilized powder of Ba-SLNs. Through the characterization indicated that the drug to amorphous state dispersed in a lipid. In vitro dissolution experiments showed Ba-SLNs compared with pure drugs had obviously sustained release. Conclusion: The technique of preparing Ba-SLN by solvent emulsification-evaporation has small particle size, high EE, and good stability, and the process is simple.
