Synthesis and identification of artificial antigen of loganin and preliminary study on its immunogenicity
10.7501/j.issn.0253-2670.2015.19.009
- Collective Name:School of Basic Medical Sciences, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Antibody titer;
Artificial antigen;
Coating antigen;
ELISA;
Immunoassay method;
Immunogenic antigen;
Loganin;
MALDI-TOF-MS;
Monoclonal antibody;
Sodium periodate oxidation method
- From:
Chinese Traditional and Herbal Drugs
2015;46(19):2870-2873
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To synthesize and identify the artificial antigen of loganin for the first time, and provide a foundation for the preparation of specific monoclonal antibody and establishment of immunoassay method. Methods: Sodium periodate oxidation method was used to synthesize immunogenic antigen (loganin-BSA) and coating antigen (loganin-OVA) of loganin. Whether loganin was conjugated with BSA and OVA or not was confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The titer and specificity of the antibody in serum of immunised mice were detected by enzyme-linked immunosorbent assay (ELISA). Results: The results of MALDI-TOF-MS indicated that loganin was conjugated to BSA. The antibody against loganin obtained from immunised-mice could bind to loganin and the titer was up to 1:40 000. Conclusion: The artificial antigen of loganin was synthesized, which can be used further in the preparation of monoclonal antibody, application in quality control of Chinese materia medica and the pharmacokinetic study of loganin in laboratory animals.
