Evaluation of consistency and difference of Polygoni Cuspidati Rhizoma et Radix pieces using total statistical moment method combined with cluster analysis and principal component analysis
10.7501/j.issn.0253-2670.2015.19.008
- Author:
Yuan-Qing WANG
1
Author Information
1. Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology
- Publication Type:Journal Article
- Keywords:
Cluster analysis;
Consistency;
Difference;
Emodin;
Emodin glycosides;
Fingerprint;
Polydatin;
Polygonum cuspidatum Sieb. et Zucc;
Principal component analysis;
Resveratrol;
Total statistical moment method
- From:
Chinese Traditional and Herbal Drugs
2015;46(19):2863-2869
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method to evaluate the consistency and difference of Polygoni Cuspidati Rhizoma et Radix from different batches. Methods: Chemical fingerprints of 11 batches of Polygoni Cuspidati Rhizoma et Radix were established by HPLC; The follow parameters, such as under curve of total quantum (AUCT), area under curve of total quantum perweight (AUCPWT), mean chromatographic retention time of total quantum (MCRTT), and variance of chromatographic retention time of total quantum (VCRTT), were also calculated using total statistical moment method. The similarities were obtained by total statistical moment method, cosine method, and correlation coefficient method. Cluster analysis (CA) and principal component analysis (PCA) were adopted to classify the samples. Results: Chemical fingerprints were established with 13 common peaks and polydatin, resveratrol, emodin glycosides, and emodin were identified. AUCT, AUCPWT, MCRTT, and VCRTTwere calculated as 1.52 × 106 AU·min, 2.31 × 103 AU·min·mL/mg, 22.0 min, and 110.0 min2, respectively. The similarities of all batches of samples were over 0.85. The samples were divided into four categories by CA or PCA. Conclusion: Chemical fingerprint combined with total statistical moment method, CA, and PCA can evaluate the consistency and difference of Polygoni Cuspidati Rhizoma et Radix, which is suitable for the quality control of Chinese materia medica.
