Isolation and expression analysis of aspartokinase gene fragment from Carthamus tinctorius
10.7501/j.issn.0253-2670.2015.20.018
- Author:
Yan-Fang WANG
1
Author Information
1. Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Aspartokinase;
Carthamus tinctorius L.;
Gene cloning;
Gene expression;
Homology
- From:
Chinese Traditional and Herbal Drugs
2015;46(20):3065-3070
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone the aspartatokinase (AK) gene from the seeds of Carthamus tinctorius (Ct, safflower) and study its expression in different developmental stages of seeds. Methods: Primers were designed according to CtAK gene segment which was selected from transcriptome sequencing results of safflower. Taking total RNA of safflower seed as template, CtAK gene was amplified by RT-PCR and connected to pEASY-T1 carrier, identified by PCR and enzyme digestion, and positive cloning was screened and sequenced. Results: The fragment of AK gene is cloned from safflower, and PCR primers of safflower are designed based on CtAK gene fragment. PCR analysis is performed in the safflower seeds of different developmental stages. The sequence of 486 bp was isolated. The expression of CtAK gene in DAF 13 in Chuan-hong 1 line is the highest. Conclusion: The gene has higher homology compared with the AK from other species.
