Structural characterization of C-glycosyl flavonoids in Scutellaria baicalensis and differentiation of isomers by UPLC/LTQ-Orbitrap-MS
10.7501/j.issn.0253-2670.2015.22.006
- Author:
Tong XIE
1
Author Information
1. Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
C-glycosyl flavonoids;
Isomers;
Scutellaria baicalensis Georgi;
Structural characterization;
UPLC/LTQ-Orbitrap-MS
- From:
Chinese Traditional and Herbal Drugs
2015;46(22):3318-3325
- CountryChina
- Language:Chinese
-
Abstract:
Objective: An UPLC-LTQ-Orbitrap-MS spectrometry method has been developed for the efficient separation and detection of C-glycosyl flavonoids in the extract of Scutellaria baicalensis differentiation of isomers. Methods: Data were acquired by Orbitrap. MS2, MS3, and MS4 data were triggered by data-dependent acquisition mode. Isomers of C-glycosyl flavonoids were distinguished by a comparison study of mass distribution. Results: A total of 19 compounds were identified as C-glycosyl flavonoids, and several isomers were successfully discriminated. Conclusion: This method is proved to be powerful for the identification of C-glycosyl flavonoids and provides the important complementary information for other flavonoid analysis.
