Imprinting equivalence research of Lonicerae Japonicae Flos and Lonicerae Flos
10.7501/j.issn.0253-2670.2019.04.007
- VernacularTitle: 金银花与山银花体外印迹等效性的研究
- Author:
Hui LIU
1
Author Information
1. College of Pharmacy, Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Chrysanthemi Flos;
Heterogeneous equivalence;
Imprinted template;
Lonicerae Flos;
Lonicerae Japonicae Flos;
Shuanghuanglian;
Supramolecular;
Total quantum statistical moment;
Yinqiaosan
- From:
Chinese Traditional and Herbal Drugs
2019;50(4):829-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore and confirm the imprinting equivalence of Lonicerae Japonicae Flos (LJF) and Lonicerae Flos (LF) based on the autonomous function of the supramolecular imprinted template, and provide a new solution for double followers dispute. Methods: Using LJF dregs, LF dregs, and Chrysanthemi Flos (CF) dregs as host molecules, and the water extract of LJF, LF, Shuanghuanglian (LJF), Shuanghuanglian (LF), Yinqiaosan (LJF), and Yinqiaosan (LF) as guest molecules, the selective absorb of three host molecules and six guest molecules was carried out. The change of fingerprint of water extract of guest molecules was determinated by HPLC. Then, the MRT and their difference value was calculated through the total quantum statistical moment method,and t-test was performed on it. Results: When the six guest molecules was absorbed by LJF and LF dregs, LJF and CF dregs, LF and CF dregs, the MRT difference value was conducted by t-test. The results were P1 = 0.94 > 0.05, P2 = 0.02 < 0.05, and P3 = 0.04 < 0.05. We can see that all guest molecules was absorbed by LJF and LF dregs. There was no significant difference in the MRT difference value. But when six guest molecules was absorbed by CF dregs. There was significant difference between CF and LJF and CF dreg in the MRT difference value. Conclusion: The statistical data indicated that similarity was existed in both “imprinted template” of LJF and LF. There was difference with CF. It is identified the exist of imprinting equivalence of LJF and LF, which is compatible with clinical medication.
