Fungal diversity and differential analysis of fungi community composition in paris polyphylla var. chinensis rhizosphere and endophytic
10.7501/j.issn.0253-2670.2019.05.030
- VernacularTitle: 重楼根际及药用部位内生真菌多样性与群落结构差异分析
- Author:
Yan WANG
1
Author Information
1. College of Life Sciences, Shaanxi Normal University
- Publication Type:Journal Article
- Keywords:
Community;
Diversity;
Endophytic fungi;
High-throughput sequencing;
Paris polyphylla Sm. var chinensis (Franch.) Hara
- From:
Chinese Traditional and Herbal Drugs
2019;50(5):1232-1237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find the microbes promoting the biosynthesis of active metabolites in host plant, which could provide theoretical support to the establishment of functional flora and discovery of key genes. Methods The ITS1 region of rhizosphere fungi and endophytic fungi from Paris polyphylla var. chinensis was sequenced by Illumina Miseq PE250 high-throughput sequencing technique, and the biological information was also analyzed. Results It was shown that the number of effective sequences for sample was 154 228, and these sequences were assigned to 408, 119, and 57 OTUs with rhizosphere soil, rhizome, and root, respectively. Conclusion It was indicated that the diversity relationship between rhizosphere fungi and endophytic fungi of Paris polyphylla var. chinensis was rhizosphere > rhizome > root. It was found that there were more Mucoromycota Phylum in the rhizome than root, and the dominant population of endophytic fungi in rhizome of Paris polyphylla var. chinensis. belongs to Roseodiscus. Ascomycota is the dominant Phylum. It is suggested that the specific fungi may be an important microbial community affecting the biosynthesis of the active products in Paris polyphylla var. chinensis.
