Effect of Duzhong Butiansu Capsule on improving spermatogenesis obstacle in mice induced by cyclophosphamide
10.7501/j.issn.0253-2670.2019.11.018
- Author:
Meng-Meng WANG
1
Author Information
1. Henan University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cyclophosphamide;
Duzhong Butiansu Capsule;
Dyszoospermia;
HO-1;
NQO1;
Nrf2/ARE signal pathway;
Reproductive protection
- From:
Chinese Traditional and Herbal Drugs
2019;50(11):2625-2631
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the reproductive protective effect of Duzhong Butiansu Capsule (DBC) by using cyclophosphamide induced spermatogenic disorder model, and explore its mechanism. Methods: The model of spermatogenic disorder was established by intraperitoneal injection of cyclophosphamide (60 mg/kg) for 5 d. Drug intervention at high and low doses (1.388 g/kg and 0.694 g/kg) was given for 4 weeks from the 8th day. The body weight and organ index of each group were measured. The pathological structure of testis was detected by HE staining. ELISA method was used to detect the levels of T, FSH, LH, MDA, SOD, GSH-Px. Western blotting and immunohistochemistry were used to analyze the expression of Nrf2/ARE signaling pathway related factors Nrf2, HO-1, NQO1, HDAC2, and p-PKC in testicular tissue. Results: Compared with the model group, DBC significantly reduced the weight of mice, increased the index of testis, epididymis and kidney, improved the pathological morphology of testis, increased the number of spermatozoa, increased the motility of sperm, decreased the rate of abnormal sperm, increased the level of T and decreasd the level of LH and FSH, increased the content of MDA, decreased the content of SOD and GSH-Px, increased the expression of Nrf2, HO-1, NQO1, HDAC2, and p-PKC protein, and increased the area of positive expression of Nrf2, HO-1 protein (P < 0.05 or P < 0.01). Conclusion: DBC can obviously improve the spermatogenic disorder induced by cyclophosphamide, and the mechanism may be related to the regulation of Nrf2/ARE signal pathway associated with oxidative stress.
