Metabolomics-based study of metabolic changes in liver tissues of senescence accelerated mouse prone 8 (SAMP8) treated with baicalein
10.7501/j.issn.0253-2670.2019.11.015
- Author:
Feng ZHOU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University
- Publication Type:Journal Article
- Keywords:
Aging;
Baicalein;
Liver damage;
Metabolomics;
SAMP8
- From:
Chinese Traditional and Herbal Drugs
2019;50(11):2604-2611
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect and explore the mechanism of baicalein on the liver of senescence-accelerated mouse prone 8 (SAMP8) based on 1H-NMR metabolomics. Methods: The protective effect of baicalein (ig) on the liver of SAMP8 mice was investigated in the present study. The mice in control group was SAMR1, the mice in model group was SAMP8, the drug treatment group was SAMP8 + baicalein. The mice in control and model group were administrated with equal amount of normal saline, and the mice in drug treatment group were administrated with 200 mg/kg baicalein. The liver tissues of mice in each group were isolated, and the damage degree of liver tissue was determined by hematoxylin-eosin (HE) staining. 1H-NMR combined with multivariate statistical analysis was used to investigate the mechanism of baicalein on liver damage in aging mice. Results: Organ index and HE staining results showed that baicalein can significantly improve liver damage in SAMP8 mice. Eight potential biomarkers were found in hepatic metabolomics analysis, mainly involving three metabolic pathways: Alanine, aspartic acid and glutamate metabolism; Glycine, serine, threonine metabolism, and inositol phosphate metabolism. Conclusion: The study of metabolites alterations in the liver tissue of SAMP8 mice would provide experimental evidence for anti-aging drug research.
