A qualitative and semi-quantitative study of Salvia miltiorrhiza aqueous extracts
10.7501/j.issn.0253-2670.2019.15.013
- VernacularTitle: 丹参水提取物成分的定性与半定量研究
- Author:
Wei-Tao ZHANG
1
Author Information
1. China Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Qualitation;
Salvia miltiorrhiza aqueous extracts;
Salvianolic acid;
Semi-quantification;
UPLC/MS
- From:
Chinese Traditional and Herbal Drugs
2019;50(15):3598-3606
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To qualitative research the composition of Salvia miltiorrhiza aqueous extracts by using Q-TOF-MS and to establish a semi-quantitative analysis method of some active components in S. miltiorrhiza aqueous extracts based on Q-Trap-MS. Methods: The chromatographic separation was performed on a Waters Acquity UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm) column with the mobile phase consisting of acetonitrile and 0.1% formic acid for gradient elution. The flow rate was 0.3 mL/min, and the column temperature was 30 ℃. In Q-TOF-MS, electrospray ionization (ESI) source was applied and operated in negative ion mode. The capillary voltage was set at 2.5 kV. The nebulization gas was set to 800 L/h at 400 ℃, and the source temperature was 100 ℃. In Q-Trap-MS, electrospray ionization (ESI) source was applied and operated in negative ion mode. The ionspray voltage was set to -4 500 V at 450 ℃. The pressure of source gas 1 (GS1, N2) and source gas 2 (GS2, N2) were both 45 psi, and the pressure of curtain gas (N2) was 20 psi. Semi-quantification analysis was performed with multiple reaction monitoring (MRM) mode. Results: A total of 26 components of S. miltiorrhiza aqueous extracts were identified, of which 15 components were common phenolic acids. The two reference substances had good linearity ranges (r ≥ 0.997 6), and the values of precision, repeatability, stability and recovery met the demand of Chinese Pharmacopoeia. The content of 11 unknown compositions was determined. Conclusion: The composition identification of S. miltiorrhiza aqueous extracts was realized with a high effective and rapid method, and a new idea for the content determination of unknown components was provided.
