In vivo imaging in tumor-bearing animals and tissue distribution of nanostructured lipid carrier modified by hyaluronic acid loaded with three components in Panax ginseng
10.7501/j.issn.0253-2670.2019.17.029
- Author:
Shuang SUN
1
Author Information
1. College of Pharmacy, Heilongjiang University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Ginsenosider Rg3;
In vivo imaging;
Laser confocal scanning microscopy;
Nanostructured lipid carriers;
Oleanolic acid;
Panax ginseng C. A. Mey.;
Tissue distribution;
Tumor targeting;
Ursolic acid
- From:
Chinese Traditional and Herbal Drugs
2019;50(17):4209-4218
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Human hepatoma SMMC-7721 cells were transplanted into nude mice to study the tissue distribution of nanostructured lipid carrier modified by hyaluronic acid (HA-OUR-NLC) loaded with three components in Panax ginseng (oleanolic acid, ursolic acid, and ginsenosider Rg3, OUR). Methods: FITC and DiR were used as fluorescent probes to dynamically monitor the HA-OUR-NLC targeted behavior of various tissues and organs through fluorescence endoscopic confocal imaging and in vivo imaging studies. Results: RUE values of oleanolic acid, ursolic acid, and ginsenosider Rg3 in tumors were significantly increased in HA-OUR-NLC group, reaching 2.51 ± 1.23, 2.27 ± 1.43, and 2.77 ± 0.25, respectively, which indicated that nanoparticles modified by hyaluronic acid could enhance drug uptake in tumors. The DiR accumulation in tumors of DiR-HA-OUR-NLC was higher than that of DiR-OUR-NLC by the visualized fluorescence of in vivo imaging. Conclusion: It indicated that nanoparticles modified by hyaluronic acid loaded with three components in P. ginseng can be enriched in the tumor site of liver cancer, which is in line with the expectation and can significantly improve the tumor targeting of the drug delivery system.
