Research on mechanism of Sini Powder in treatment of depression based on network pharmacology
10.7501/j.issn.0253-2670.2019.20.022
- VernacularTitle: 基于网络药理学四逆散治疗抑郁症的作用机制探讨
- Author:
Yang ZONG
1
Author Information
1. Affiliated Hospital of Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Depression;
Dopaminergic synapse signaling pathway;
IL-17 signaling pathway;
Network pharmacology;
Neuroactive ligand-receptor interaction signaling pathway;
Sini Powder
- From:
Chinese Traditional and Herbal Drugs
2019;50(20):4995-5002
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of depression by Sini Powder. Methods: The chemical composition and targets of Bupleuri Radix, Paeonia Radix Alba, Aurantii Fructus Immaturus, and Glycyrrhizae Radix et Rhizoma were searched by the analysis of traditional Chinese medicine system pharmacology platform (TCMSP). Depression related genes were screened from OMIM, TDD, Drugbank, and Digsee multiple databases. The target corresponding genes were searched through UniProt, GeneCards, and PubMed database query and then Cytoscape 3.2.1 was used to build compound-targets (genes) networks, protein interaction (PPI) filter core targe; At last, the enrichment of gene ontology (GO) function analysis by DAVID based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was carried out, and the mechanism of its action research was predicted. Results: The compound-target network contained 121 compounds and the corresponding 259 targets, and the key targets involved PTGS2, CALM1, ESR1, HSP90AA1, AR, etc. The PPI core network contained 15 proteins, key proteins involved in CASP3, CHRM2, CYP3A4, and etc. The function enrichment analysis of GO was 375 (P < 0.05), of which there were 307 biological processes (BP), and 37 related items of cell composition (CC), and 31 molecular function (MF) items. 37 related items of cell composition (CC), and 31 molecular function (MF) items. There were 37 signal pathways (P < 0.05) in KEGG pathway enrichment screening, involving neuroactive ligand-receptor interaction, dopaminergic synapse, IL-17 signaling pathway and so on. Conclusion: The active components in Sini Powder play an antidepressant role by acting on 15 key targets such as CASP3, CHRM2, DRD1 to regulate multiple signaling pathways.
