Self-assembly method of preparation of puerarin chitosan/sodium alginate oral nanoparticles by Box-Behnken response surface method
10.7501/j.issn.0253-2670.2019.23.010
- Author:
Jie YAN
1
Author Information
1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Box-Behnken;
Drug loading;
Encapsulation efficiency;
Nanoparticle;
Particle size;
Polydispersity index;
Puerarin
- From:
Chinese Traditional and Herbal Drugs
2019;50(23):5706-5713
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen the optimal formulation of puerarin nanoparticles (Pur-NPs) by self-assembly of chitosan/alginate by Box-Behnken response surface method. Methods: Puerarin chitosan/sodium alginate nanoparticles (Pur-CS/SA-NPs) were prepared by self-assembly method. Taking chitosan concentration, chitosan pH, sodium alginate concentration, stirring speed, stirring time, ultrasonic power, and dosage as investigation factors, the encapsulation rate, drug loading, particle size, and polydispersity index (PDI) were used as evaluation index to investigate the optimal prescriptions using the Box-Behnken response surface design method, and the optimal formulation was characterized and released in in vitro experiments. Results: The optimal formulation from the optimized self-assembly method was as following: chitosan concentration of 0.45 mg/mL, sodium alginate concentration of 0.07 mg/mL, chitosan pH of 4.33, and rotation speed of 316.49 r/min. For the convenience of experimental operation, the most optimal prescription was determined to be chitosan concentration of 0.45 mg/mL, sodium alginate concentration of 0.07 mg/mL, chitosan pH of 4.3, and rotation speed of 300 r/min. The results were verified by three parallel experiments, and the encapsulation efficiency, drug loading, average particle diameter, and PDI of obtained nanoparticles were (89.056 ± 1.680)%, (44.528 ± 0.840)%, (208.327 ± 1.870) nm and 0.131 ± 0.006, respectively. After characterization, the morphology of the nanoparticles was intact. It can be seen from the in vitro release experiments that the equation fitted by the nanoparticles was Higuchi model, and there was no burst release during the release process, indicating that the nanoparticles were released well in vitro. Conclusion: The prescription of puerarin chitosan/sodium alginate nanoparticles was optimized by Box-Behnken response surface method. The model was evaluated by average particle size, PDI, encapsulation efficiency and drug loading, and the characterization results and in vitro release test showed that the model had good predictability.
