Trienomycin J, a new ansamycin from deep-sea derived bacterium Ochrobactrum sp.
10.7501/j.issn.0253-2670.2019.23.003
- Author:
Cong WANG
1
Author Information
1. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities
- Publication Type:Journal Article
- Keywords:
Ansamycins;
Breast cancer cells cytotoxicity;
Deep-sea derived bacterium;
Ochrobactrum sp.;
Trienomycin J
- From:
Chinese Traditional and Herbal Drugs
2019;50(23):5661-5665
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the cytotoxic natural products from the deep-sea derived Ochrobactrum sp. OUCMDZ-2164. Methods: The isolations and purifications of compounds were performed by means of column chromatography over silica gel and Sephadex LH-20 as well as HPLC. Their structures were elucidated through the analysis of UV, IR, MS, NMR and ECD spectra. The cytotoxicities against MCF-7, A549 and K562 cells were evaluated by MTT and CCK-8 methods. Results: From the fermentation broth of Ochrobactrum sp. OUCMDZ-2164, we isolated and identified four compounds (1-4). Compound 1 was identified as a new ansamycin and named trienomycin J, and the structures of 1-4 were identified as 3-O-demethyltrienomycinol, flazin, flazin-3- carboxylic acid and thymine, respectively. Compound 1 showed cytotoxic effect on the MCF-7 cells with 61.5% inhibition rate at 10 μmol/L. Conclusion: Compound 1 was a new ansamycin named trienomycin J, with cytotoxic activity against human breast cancer cells (MCF-7).
