Structural characterization and anti-complement activity of polysaccharides from Glechomae Herba
10.7501/j.issn.0253-2670.2020.19.010
- VernacularTitle: 连钱草多糖的结构表征及抗补体活性研究
- Author:
Ting TAN
1
Author Information
1. National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Anti-complement activity;
Glechomae Herba;
Polysaccharide;
Purification;
Structural characterization
- From:
Chinese Traditional and Herbal Drugs
2020;51(19):4916-4924
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate and purify the homogeneous polysaccharides from Glechomae Herba through the guidance of anti-complement activity, and study their structures. Methods: Crude polysaccharides from Glechomae Herba were extracted by water extraction and alcohol precipitation, then homogeneous polysaccharides were separated by DEAE-52 and Sephadex G-100 column chromatography. The structures of homogeneous polysaccharides were characterized by HPGPC, IR, GC, methylation and NMR, and their anti-complement activities were also determined. Results: Two homogeneous polysaccharides GLP-1 and GLP-2 were obtained with the molecular weight of 5 370 and 20 040. They were both heteropolysaccharides composed of arabinose and galactose with the ratio of 1:6.3 and 1:4.9, respectively. Conclusion: The structures of GLP-1 and GLP-2 further elucidate the pharmacodynamic basis of their anti-complement activities and provide basis for screening natural complement inhibitors.
