Effects of celastrol on proliferation and aerobic glycolysis of gastric cancer cells
- Author:
Ke LI
1
Author Information
- Publication Type:Journal Article
- Keywords: Aerobic glycolysis; Celastrol; Cell proliferation; Gastric cancer
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2019;40(4):658-663
- CountryChina
- Language:Chinese
- Abstract: Objective: To explore the effects of celastrol on the proliferation and aerobic glycolysis of gastric cancer cell line BGC-823. Methods: After treatment with celastrol, the proliferation of BGC-823 cells was detected by CCK8 assay and clone formation assay; cell apoptosis and cell cycle distribution were detected by flow cytometry. Glucose utilization, lactic acid production, hexokinase (HK) activity and lactate dehydrogenase (LDH) activity were detected by spectorphotometry; Western blot was conducted to detect the expressions of glucose transporter 1(GLUT1), hexokinase Ⅱ (HKⅡ), pyruvate kinase M2 (PKM2), and LDH in BGC-823 cells. Results: Exposed to celastrol, BGC-823 cells' proliferation was inhibited, cell apoptosis was induced, and cell cycle was blocked in G2 phase. Glucose utilization and lactate production were decreased. Moreover, celastrol downregulated the expressions of GLUT1, HKII, and LDH protein as well as the activities of HK and LDH enzyme. Conclusion: Celastrol can inhibit the proliferation of BGC-823 cells and it may be related to the inhibition of aerobic glycolysis.
