HO-1 increases the proliferation ability of pancreatic cancer cells
- Author:
Jie JIANG
1
Author Information
- Publication Type:Journal Article
- Keywords: HO-1; Pancreatic cancer; Proliferation
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2019;40(4):517-520
- CountryChina
- Language:Chinese
- Abstract: Objective: To study the potential effect of HO-1 on the proliferation ability of pancreatic cancer cells. Methods: The mRNA expression of HO-1 in pancreatic cancer cells was detected by Real-time PCR.Immunofluorescence and Western blot analysis were made to detect the protein expression of HO-1. The gene transfection technology was used to construct a high-expression HO-1 in Miapaca-2 cells, and low-expression HO-1 in Panc-1 cells. MTT assay was carried out to examine the effect on the proliferation ability of pancreatic cancer cells. Results: The expression of HO-1 existed in pancreatic cancer cells. We successfully constructed the high- and low-expression HO-1 in pancreatic cancer cell lines. The results showed that ZnPPIX and Panc-1-Si-HO-1 could significantly inhibit the proliferation ability of Panc-1 cells. In Miapaca-2 cells, ZnPPIX could significantly inhibit the proliferation ability of pancreatic cancer cells, while Miapaca-2-Sh-HO-1 could significantly promote the proliferation ability of cancer cells. Conclusion: HO-1 can significantly enhance the proliferation ability of pancreatic cancer cells.
