Upregulation of lncRNA-TDRG1 correlates with clinical progression and unfavorable prognosis of cervical cancer
- Author:
Yang FAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Cervical cancer; LncRNA-TDRG1; Long noncoding RNA; Prognosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2019;40(6):888-892
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the expression of long non-coding RNA testis developmental related gene 1 (lncRNA-TDRG1) in cervical cancer tissues and its correlation with clinical progress and prognosis. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine the relative expression of lncRNA-TDRG1 in cervical cancer tissues (n=60) and adjacent normal tissues (n=60). We also compared lncRNA-TDRG1 expression in different cervical cancer patients of different age, FIGO stages, cancer cell differentiation, and lymph node metastasis. Furthermore, four kinds of cervical cancer cells (SiHa, HeLa, CaSki, and C-33A) and a normal cervical cell (Ect1/E6E7) were used to identify the different expression of lncRNA-TDRG1 with qRT-PCR. Chi-square test was performed to analyze the relationship between lncRNA-TDRG1 expression and clinicopathological characteristics in the cervical cancer patients. Survival curves were drawn with Kaplan-Meier method and analyzed with log-rank test. In addition, we also carried out univariate and multivariate Cox regression analyses of overall survival in 60 cervical cancer patients. Results: lncRNA-TDRG1 was highly expressed in cervical cancer tissues as compared with adjacent normal tissues. And cervical cancer cells also showed a higher expression of lncRNA-TDRG1 than normal cervical cells. The expression of lncRNA-TDRG1 in cervical cancer exhibited obvious relationship with cancer cell differentiation (moderate and well vs. poor, P=0.019) and lymph node metastasis (yes vs. no, P=0.037). Meanwhile, patients with a higher expression of lncRNA-TDRG1 had a worse overall survival (P<0.05). Univariate and multivariate Cox regression analyses of overall survival indicated that lncRNA-TDRG1 was an independent risk factor for poor prognosis of cervical cancer patients (P=0.006). Conclusion: lncRNA-TDRG1 plays a very important role in the progression and prognosis of cervical cancer, and may serve as a biomarker for the prognosis of patients with cervical cancer.
