Linc00460 promotes cancer by down-regulating miR-654-3p in esophageal cancer
- Author:
Zhen-Wei YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Esophageal cancer; Linc00460; Lnc RNA; MiR-654-3p; Proliferation
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2019;40(6):871-876
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the role and expressions of linc00460 and miR-654-3p in esophageal cancer so as to further investigate their role in the prognosis and biology of esophageal cancer. Methods: We divided 121 patients with esophageal cancer into linc00460 high expression and low expression groups. Chi-square test was used for differential expression analysis and log-rank test and COX model were used for survival analysis. R was used for nomogram plots with C-index for internal validation. CCK-8 was used for proliferation tests of linc00460 and miR-654-3p. Dual luciferase reporter gene assay was used to investigate the association between linc00460 and miR-654-3p expression levels. Results: Linc00460 was highly expressed in esophageal cancer while miR-654-3p was lowly expressed. Chi-square tests indicated that the expression of linc00460 was related to the expression of miR-654-3p. Log-rank indicated that the expressions of linc00460 and miR-654-3p were associated with the prognosis of esophageal cancer patients. CCK-8 indicated that linc00460 promoted the proliferation and it was directly bound to miR-654-3p to exert its oncogenic function. Conclusion: Linc00460 and miR-654-3p expression profiles were associated with the prognosis of esophageal cancer patients, and linc00460 promoted cancer progression through downregulating miR-654-3p.
