KLF5 suppresses TRAIL-induced cell apoptosis in PC-3 prostate cancer cells

Author: Chao YANG ¹
Author Information

1. Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University
Publication Type:Journal Article
Keywords: Apoptosis; Cellular fas-associated death domain-like interleukin-1β converting enzyme inhibitory protein (c-FLIP); Krüppel-like factor 5 (KLF5); Prostate cancer; Tumor necrosis factor-related apoptosis inducing ligand (TRAIL)
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2020;41(2):206-209
CountryChina
Language:Chinese
Abstract: Objective: To explore the possibility of promoting tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced apoptosis in prostate cancer PC-3 cell by inhibiting Krüppel-like factor 5 (KLF5). Methods: MTT assay, flow cytometry, Western blot assay and qRT-PCR assay were deployed to detect the cell viability, apoptosis and apoptotic markers in KLF5-inhibited and TRAIL-induced PC-3 cells. Results: After KLF5 was inhibited in TRAIL-induced PC-3 cells, cell viability reduced, apoptosis enhanced, the expressions of DR4 and DR5 increased while the expression of cellular fas-associated death domain-like interleukin-1β converting enzyme inhibitory protein (c-FLIP) decreased. Conclusion: Inhibiting KLF5 suppresses cell viability by promoting TRAIL-induced apoptosis in prostate cancer cell PC-3. It may be a potential means to treat hormone-insensitive prostate cancer.