Intrauterine high-nutrition induced immunoreaction abnormality in monocytes and nature killer cells in offspring
10.3969/j.issn.1674-8115.2018.07.006
- Author:
Hui LIN
1
Author Information
1. International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine
- Publication Type:Journal Article
- Keywords:
Cytokine;
Macrosomia;
Monocyte;
Nature killer cell;
Umbilical cord blood
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2018;38(7):753-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective • To explore the effect of intrauterine high-nutrition on cytokine expression in umbilical cord blood-derived monocytes and nature killer (NK) cells. Methods • Samples of umbilical cord blood were collected from macrosomia (n=10) and infants with normal birthweight (n=18). Monocytes and NK cells were obtained by magnetic sorting. Purified monocytes were stimulated by lipopolysaccharide (LPS), while purified NK cells were stimulated by phorbol myristate acetate (PMA) and ionomycin (ION). The expression of cytokines was detected by real-time PCR and ELISA. Results • There was no significant difference in cytokine expression in the monocytes treated with PBS between macrosomia and infants with normal birthweight. However, dampened responses following LPS stimulation were observed in macrosomia group, including the mRNA and protein expression of IL-1α, IL-1β, TNF-α, IL-10 and CCL5. Moreover, mRNA and protein expression of TNF-α were significantly higher in NK cells from macrosomia group, while no significant difference in IFN-γ was found. Conclusion • Intrauterine high-nutrition might result in dampened response in monocytes following LPS stimulation and exaggerated expression of TNF-α in NK cells.
