Mechanism of TWEAK promoting macrophage-derived exosomal miR-7 to epithelial ovarian cancer cell through regulating Dicer
10.3969/j.issn.1674-8115.2018.07.004
- Author:
Xing-Di QIU
1
Author Information
1. Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Gynecology Oncology
- Publication Type:Journal Article
- Keywords:
Dicer;
Epithelial ovarian cancer;
Exosome;
Macrophage;
Tumor necrosis factor like weak inducer of apoptosis (TWEAK)
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2018;38(7):740-744
- CountryChina
- Language:Chinese
-
Abstract:
Objective • To investigate the mechanism of tumor necrosis factor like weak inducer of apoptosis (TWEAK) promoting macrophagederived exosomal miR-7 to epithelial ovarian cancer (EOC). Methods • The expression of Dicer was detected by real-time PCR and Western blotting in TWEAK-stimulated macrophages. The expression of miR-7 was detected by real-time PCR in the macrophage and macrophage-derived exosome after silencing Dicer in macrophage. While in Dicer-overexpressing macrophage, real-time PCR was used to detect the expression of miR-7. Then TWEAK was used to stimulate the macrophage, and the expression of miR-7 in the macrophage and macrophage-derived exosome was detected by real-time PCR. The expression of key proteins in the nuclear factor-κB (NF-κB) pathway was detected by Western blotting in TWEAK-stimulated macrophage. After pretreatment of NF-κB inhibitor, Western blotting was used to detect the expression of Dicer and key proteins in the NF-κB pathway in TWEAKstimulated macrophage. Results • The expression of Dicer in macrophage was down-regulated after TWEAK stimulating. The expression of miR-7 was up-regulated in Dicer-silencing macrophage and macrophage-derived exosome. While the expression of miR-7 was down-regulated in the macrophage and the macrophage-derived exosome in Dicer-overexpressing macrophage after TWEAK stimulating. The expression of key proteins in the NF-κB pathway in macrophage was also up-regulated after TWEAK stimulating. After inhibition of NF-κB signaling pathway, the expression of Dicer was not significantly changed in TWEAK-stimulated macrophage compared to the control group. Conclusion • TWEAK can active NF-κB pathway and inhibit the expression of Dicer to promote macrophage-derived exosomal miR-7 to EOC cells.
