Small peptide KR-12-a5 promotes the osteogenic differentiation of human bone marrow mesenchymalstem cells
10.3969/j.issn.1674-8115.2018.08.005
- Author:
Hui LI
1
Author Information
1. Department of Bone and Joint Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine
- Publication Type:Journal Article
- Keywords:
Antimicrobial peptides (AMPs);
Human bone marrow mesenchymal stem cells (HBMSCs);
KR-12-a5;
Osteogenic differentiation
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2018;38(8):881-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of KR-12 analog, KR-12-a5, on osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). Methods: Osteogenic differentiation-related experiments were performed using HBMSCs. KR-12-a5 was used as additional stimulation under osteogenesis inducing environment. By alkaline phosphatase (ALP) staining assay, ALP quantitative assay, alizarin red staining and elution quantitative analysis, and real-time quantitative polymerase chain reaction (RT-qPCR), the effect of KR-12-a5 on osteogenic differentiation of HBMSCs was verified. Results: As the concentration of KR-12-a5 increased, the staining intensity of ALP and alizarin red also increased. The strongest staining effect was exhibited at the highest concentration (50 μg/mL) of KR-12-a5. Quantitative tests showed similar results. After 3 days of stimulation with KR-12-a5, the mRNA level of RUNX2 increased in a dose-dependent manner. On the 7th day, the expression levels of ALP, COL1A1, BSP and BMP2 in the KR-12-a5-treated group showed significant changes. On the 14th day, OSX, OCN and OPN levels increased significantly. Conclusion: KR-12-a5 promoted the osteogenic differentiation of HBMSCs in a dose-dependent manner.
