Evaluation of SDF-1 complex with CGF applied to canine dental-pulp-like tissue regeneration
10.3969/j.issn.1674-8115.2019.10.008
- Author:
Lu QIAO
1
Author Information
1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Department of Pediatric Dentistry, School of Stomatology, Tongji University
- Publication Type:Journal Article
- Keywords:
Concentrated growth factor (CGF);
Immature permanent teeth;
Pulp regeneration;
Stromal cell-derived factor-1 (SDF-1)
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2019;39(10):1148-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the feasibility of immature permanent teeth pulp regeneration with a new method that utilizes the integration of concentrated growth factor (CGF) as a scaffold and stromal cell-derived factor-1 (SDF-1). Methods: Canine dental pulp cells (DPC) were isolated and cultured in vitro. Then the effects of SDF-1 and CGF were observed on DPC proliferation and differentiation. The pulpless model was established on the beagle’s immature incisors which were divided into four groups: natural pulp (A), empty cannel (B), CGF-filling (C) and SDF-1/CGF-filling (D). After 10 weeks, specimens were checked by imaging examination, RT-PCR and histological observation. Results: CGF extraction (CGFe) induced DPC proliferation while the combination of SDF-1 and CGFe enhanced this effect and also facilitated odontogenic and angiogenic differentiation of DPC. According to imaging examination, the apex growth of all four groups was in varying degrees. RT-PCR indicated the expressions of odontogenesis and angiogenesis related genes in group D were higher than those in group C. Besides, neonatal dentin and dental-pulp-like tissue were observed inside the canal of both group C and D, while only cementum-like tissue existed around root apex of group B. Conclusion:SDF-1 plays an important role in driving the process of pulp-like-tissue regeneration of immature permanent teeth by using CGF as an effective scaffold.
