Effect of Pingyangmycin on rat spleen tissue and its mechanism
- Author:
Ping LI
1
Author Information
1. Department of Otohinolaryngology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):178-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of Pingyangmycin in treatment of cavernous hemangioma using rat spleen as a model. Methods: The pathological changes of rat spleen were observed on different time points after Pingyangmycin was injected into the rat spleen. Cell apoptosis was verified with TUNEL staining and transmission electron microscope; the expression of apoptosis-related protein caspase-3 was detected with immunohistochemical method and image analysis system. Results: Injecting saline into rat spleen caused no damage to the spleen. Slight swelling was observed on d 2 and d 5 after Pingyangmycin was injected and the spleen became shrunk (with damaged-splenic sinus) on d 8 and d 14. Apoptotic cells were found on different days after Pingyangmycin injection under transmission electron microscope. TUNEL staining indicated that apoptotic cells existed extensively in endothelial cells of splenic sinus and others splenic cells. Apoptosis rate increased with time during a certain period of time after Pingyangmycin injection and caspase-3 expression increased gradually with the increase of apoptosis rate. Conclusion: Pingyangmycin causes n ecrosis of spleen mainly through inducing apoptosis; it can also cause necrosis and slight fibroblast cell hyperplasia. The apoptosis induction process is dependent on caspase-3 activation process.
