Effects of 3 carbohydrates on CAP10 promoter activity of Cryptococcus neo formans capsule-associated gene
- Author:
Zhi-Yu ZHAO
1
Author Information
1. Department of Dermatology and Venereology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):132-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of glucose, mannose and galactose on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene. Methods: Yeast cells were transfected with plasmid containing a 9 51 by length of 5′ upstream flanking sequence of CAP10 coding region and a reporter gene, chloramphenicol acetyl transferase (CAT); the transfectants were treated with different concentrations(10,20,40,60,80 mg/ml) of glucose, mannose and galactose. CAT activity was assessed by ELISA method and CAT activities of different groups were compared. Results: Different concentrations of glucose and mannose had no obvious influence on CAT activity; different concentrations of galactose had obvious influence on CAT activity and the influence was positively dependent with its dose within the experimental concentration range. Conclusion: Glucose and mannose have no obvious effect on the activity of CAP10 promoter; galactose has obvious inductive effect on activity of CAP10 promoter, suggesting that CAP10 gene might be related with galactose metabolism.
