Therapeutic effects of tumor-selective replication-competent adenovirus carrying human endostatin gene on ovarian cancer: An in vivo study in mice
- Author:
Xiong CHEN
1
Author Information
1. Department of Obstetrics and Gynecology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(7):750-754
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibitory effect of tumor-selective replication-competent adenovirus-mediated human endostatin(CX-hE) on transplanted ovarian cancer (OV-90) in nude mice. Methods: BALB/c nude mice were inoculated subcutaneously with OV-90 cells to establish the animal model bearing human ovarian cancer. Eighteen nude mice bearing cancer were divided into 3 groups to receive intratumoral injection of PBS, CX-hE or Ad-hE, 1/2 d, 5 times. Then their livers were harvested for pathologic examination. Another 15 nude mice were divided into 3 groups to receive single intratumoral injection of CX-hE, Ad-hE or ONYX-015. Venous blood was collected on day 1,3 and 7 after injection for hEndo measurement by ELISA. The tumors were harvested for pathologic examination and immunohistochemical staining. Results: The tumors grew more slowly in CX-hE group and their sizes were markedly smaller than those of Ad-hE group(P<0.05) and PBS group(P<0.01). Endostatin levels were detected in the sera of nude mice in all single injection groups, and the endostatin expression in CX-hE group increased as time passing by. The endostatin level in CX-hE treated group was much higher(P<0.01) and increased faster than that in Ad-hE treated group. Immunohistochemical staining for Hexon of adenovirus capsid showed more positive tumor cells in the tumor tissues treated with CX-hE. Immunohistochemical staining for vWF revealed a decreased microvessel density in the tumor tissues treated with CX-hE. Conclusion: CX-hE and Ad-hE can inhibit tumor growth and the effect of CX-hE is superior to that of Ad-hE. There is no growth halt or disappearance of tumors in all groups.
