High-performance liquid chromatography/tandem mass spectrometry for determination of cyclosporin a level in whole blood
- Author:
Bin WANG
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(8):899-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a high performance liquid chromatography/tandem mass spectrometry (HPLC-MS) method for determination of cyclosporin A level in the whole blood. Methods: A Hypersil C8 column (65 °C, 4.6 mm × 150 mm, 5 μm) was used with a mobile phase of acetonitrile-acetic acid (pH 4.50) 70: 30 at a flow rate of 1.0 ml · min-1. Atomospheric pressure electronic spray ionization was used to quantify cyclosporin A, with the capillary voltage being 4 000 V, the flow of drying gas being 8.0 L · min-1, nebulizer pressure being 30 psi, and fragment voltage being 140 V. Results: The calibration curve of cyclosporin A was linear in a range of 26.68-1 067 μg · L-1: y=0.001 1x-0.004 6(r=0.9993). The lowest limit of detection was 4.35 μg · L-1. The method recoveries of low, medium and high concentrations cyclosporin A were (89.81±4.91)%, (104.5±3.73)%, and (95.17±2.33)%, respectively; the extraction recoveries of 3 concentrations were (77.64.±4.38)%, (78.01±3.79)%, and (83.02±1.59)%, respectively. The intra-day and inter-day RSD was less than 6%, and the RSD was less than 5%'in 150 days. Conclusion: The present method is fast, sensitive and selective. It is suitable for clinical monitoring of cycolsporin A.
