Inhibitory effects of dexamethasone on growth of human prostate cancer cell line PC-3: The possible mechanism
- Author:
Yan WANG
1
Author Information
1. Department of Pathophysiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(8):885-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the regulatory effect of dexamethasone on NF-κB and its target gene cyclinD1 in human prostate cancer cell line PC-3, so as to explore the anti-proliferation mechanism of dexamethasone. Methods: The inhibitory effects of dexamethasone on growth of PC-3 cells were determined by MTT. The basic transcriptional activity of NF-κB in many kinds of tumor cells and the effects of dexamethasone on the activation of PC-3 cell NF-κB were determined by reporter gene assay. The expression of cyclinD1 protein was determined by Western blots. Results: Dexamethasone inhibited the growth of PC-3 cells and NF-κB was constitutively activated in the PC-3 cells. Dexamethasone also inhibited the activation of NF-κB and down-regulated the expression of cyclinD1 protein in PC-3 cells. Conclusion: Dexamethasone can inhibit cell growth of prostate cancer cell line PC-3, which might be related to the inhibitory effect of dexamethasone on the transcriptional activation of NF-κB and cyclinD1 protein.
